Journal
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Immunologie et infection
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Development of pHluorin2-Based Sensor for Glycosomal pH in Trypanosoma brucei
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Sample Preparation for Flow Cytometry to Measure pHluorin2 Fluorescence in Living Trypanosoma brucei
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Sample Preparation for Flow Cytometry to Measure pHluorin2 Fluorescence in Living Trypanosoma brucei

Sample Preparation for Flow Cytometry to Measure pHluorin2 Fluorescence in Living Trypanosoma brucei

Development of pHluorin2-Based Sensor for Glycosomal pH in Trypanosoma brucei

DOI:
10.3791/201368-v

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00:54 min

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January 19, 2024

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, , , , , ,
1Department Chemistry and Biochemistry,Brigham Young University, 2Eukaryotic Pathogens Innovation Center,Clemson University, 3Department of Genetics and Biochemistry,Clemson University, 4Department of Physics and Astronomy,Clemson University

Tags

Flow CytometryPHluorin2Trypanosoma BruceiSample PreparationTetracyclineDoxycyclineCell CultureCentrifugationPBSGlucosePropidium IodideThiazole RedLive/dead Staining

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