Rivista
/
Neuroscienze
/
Mouse Brain Histone Modification Quantification Using Flow Cytometry
/
Flow Cytometry and Data Analysis of Murine Brain Histone Post-Translational Modifications
JoVE Journal
Neuroscienze
This content is Free Access.
JoVE Journal Neuroscienze
Flow Cytometry and Data Analysis of Murine Brain Histone Post-Translational Modifications

Flow Cytometry and Data Analysis of Murine Brain Histone Post-Translational Modifications

DOI:
10.3791/200982-v

•

02:23 min

•

September 15, 2023

•

, ,
1Department of Biochemistry and Molecular Biology, Djavad Mowafaghian Centre for Brain Health,University of British Columbia, 2Graduate Program in Neuroscience, Djavad Mowafaghian Centre for Brain Health,University of British Columbia

Tags

Flow CytometryMurine BrainHistone Post-translational ModificationsAntibody PanelCompensation MatrixRainbow Fluorescent BeadsPhotomultiplier Tube VoltageCompensation BeadsSide ScatterForward ScatterSinglet CellsFMOMicrogliaP2RY12Protein ExpressionMedian Fluorescence Intensity (MFI)LipopolysaccharideHistone 3 Lysine 27 AcetylationHistogram Analysis

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Author Spotlight: Enhancements in Gene Expression Regulation Research

Mouse Brain Dissection for Microglia Isolation

Mouse Brain Immune-Enriched Fragment Isolation for Histone PTM Quantification

Mouse Brain Enriched Immune Pellet Plate Staining Method for Histone Quantification

Flow Cytometry and Data Analysis of Murine Brain Histone Post-Translational Modifications

Read Article