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Mouse Brain Histone Modification Quantification Using Flow Cytometry
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Flow Cytometry and Data Analysis of Murine Brain Histone Post-Translational Modifications
JoVE Journal
Neurowissenschaften
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JoVE Journal Neurowissenschaften
Flow Cytometry and Data Analysis of Murine Brain Histone Post-Translational Modifications

Flow Cytometry and Data Analysis of Murine Brain Histone Post-Translational Modifications

Mouse Brain Histone Modification Quantification Using Flow Cytometry

DOI:
10.3791/200982-v

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02:23 min

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September 15, 2023

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, ,
1Department of Biochemistry and Molecular Biology, Djavad Mowafaghian Centre for Brain Health,University of British Columbia, 2Graduate Program in Neuroscience, Djavad Mowafaghian Centre for Brain Health,University of British Columbia

Tags

Flow CytometryMurine BrainHistone Post-translational ModificationsAntibody PanelCompensation MatrixRainbow Fluorescent BeadsPhotomultiplier Tube VoltageCompensation BeadsSide ScatterForward ScatterSinglet CellsFMOMicrogliaP2RY12Protein ExpressionMedian Fluorescence Intensity (MFI)LipopolysaccharideHistone 3 Lysine 27 AcetylationHistogram Analysis

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