Journal
/
Neurosciences
/
Mouse Brain Histone Modification Quantification Using Flow Cytometry
/
Flow Cytometry and Data Analysis of Murine Brain Histone Post-Translational Modifications
Journal JoVE
Neurosciences
This content is Free Access.
Journal JoVE Neurosciences
Flow Cytometry and Data Analysis of Murine Brain Histone Post-Translational Modifications

Flow Cytometry and Data Analysis of Murine Brain Histone Post-Translational Modifications

67 Views

•

02:23 min

•

September 15, 2023

DOI:

10.3791/200982-v

DOI:
10.3791/200982-v

•

02:23 min
September 15, 2023

•

1 Views
, ,
1Department of Biochemistry and Molecular Biology, Djavad Mowafaghian Centre for Brain Health,University of British Columbia, 2Graduate Program in Neuroscience, Djavad Mowafaghian Centre for Brain Health,University of British Columbia

Tags

Flow CytometryMurine BrainHistone Post-translational ModificationsAntibody PanelCompensation MatrixRainbow Fluorescent BeadsPhotomultiplier Tube VoltageCompensation BeadsSide ScatterForward ScatterSinglet CellsFMOMicrogliaP2RY12Protein ExpressionMedian Fluorescence Intensity (MFI)LipopolysaccharideHistone 3 Lysine 27 AcetylationHistogram Analysis

Read Article

Cite this Article

Flow Cytometry and Data Analysis of Murine Brain Histone Post-Translational Modifications. J. Vis. Exp. (Pending Publication), e200982, doi:10.3791/200982 (2023).

Download .ris file

Copy

Share Video

.

Copy