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Mensuração em Tempo Real do Perfil Bioenergético Mitocondrial de Neutrófilos
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Immunologie et infection
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Journal JoVE Immunologie et infection
Real-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils

Mensuração em Tempo Real do Perfil Bioenergético Mitocondrial de Neutrófilos

1,367 Views

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09:39 min

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June 02, 2023

DOI:

10.3791/64971-v

DOI:
10.3791/64971-v

•

09:39 min
June 02, 2023

•

16 Views
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1Department of Immunology, School of Medicine,UConn Health, 2Arthritis and Clinical Immunology Program,Oklahoma Medical Research Foundation, 3Department of Microbiology and Immunology,University of Oklahoma Health Science Center

Summary

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Descrevemos protocolos stepwise medindo a respiração mitocondrial de neutrófilos humanos e camundongos e células HL60 usando o analisador de fluxo extracelular metabólico.

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Cite this Article

Pulikkot, S., Zhao, M., Fan, Z. Real-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils. J. Vis. Exp. (196), e64971, doi:10.3791/64971 (2023).

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