Specker, J. T., Smith, A. B., Keenan, O., Zackular, J. P., Prentice, B. M. Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection. J. Vis. Exp. (189), e64200, doi:10.3791/64200 (2022).