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评估肺炎消退的实验模型
Journal JoVE
Immunologie et infection
This content is Free Access.
Journal JoVE Immunologie et infection
Experimental Model to Evaluate Resolution of Pneumonia
DOI:

09:49 min

February 17, 2023

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Chapitres

  • 00:00Introduction
  • 01:11Bacteria Thawing for Intratracheal Instillation
  • 02:00Intratracheal Instillation of Live Bacteria
  • 03:44Bronchoalveolar Lavage (BAL) and Lung Harvest
  • 05:55Bronchoalveolar Lavage Processing
  • 06:38Lung Processing for Single Cell Suspension
  • 08:01Results: Experimental Model to Evaluate Resolution of Pneumonia
  • 09:29Conclusion

Summary

Traduction automatique

该手稿描述了小鼠肺炎感染模型的建立和损伤消退的相应表征,以及培养细菌和气管内滴注的方法。还描述了一种使用高维流式细胞术来评估免疫景观的新方法。

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