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Lysinduceret in situ transmissionselektronmikroskopi til observation af interaktionen mellem flydende og blødt stof
Journal JoVE
Ingénierie
Author Produced
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Journal JoVE Ingénierie
Light-Induced In Situ Transmission Electron Microscopy for Observation of the Liquid-Soft Matter Interaction
DOI:

05:33 min

July 26, 2022

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Chapitres

  • 00:04Introduction
  • 00:41Encapsulation of Bacteria with the Photosensitizer
  • 02:07In Situ TEM Observations of Bacterial Cells in Liquid–Electron Dose Effect
  • 02:50In Situ TEM Observations of Light‐Induced Processes Between Bacterial Cells and Photosensitizer
  • 03:51Results: TEM Images of S. aureus Encapsulated with Methylene Blue Between Two Carbon Films and the Unfavorable Effects That May Occur During Imaging
  • 04:47Conclusion

Summary

Traduction automatique

Den nuværende protokol beskriver transmissionselektronmikroskop (TEM) modifikationer med et lysbelysningssystem, fremstilling af flydende celler og in situ TEM-observationer af lysinducerede interaktioner mellem bakterieceller og en fotosensibilisator. Prøveforberedelsesmetoderne, elektronstråleskader og billeddannelse diskuteres også.

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