Targeted Laser Ablation in the Embryo of <em>Saccharina latissima</em>

00:04Introduction
00:47Image Acquisition for Selecting Embryos for Ablation and Monitoring Subsequent Growth
01:31Laser Calibration
02:48Laser Ablation
04:21Monitoring the Growth of Irradiated Embryos
04:49Results: Effect of Local Cellular Laser Ablation on the Saccharina latissima Embryo
05:46Conclusion

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The destruction of specific cells in the embryo is a powerful tool for studying cellular interactions involved in cell fate. The present protocol describes techniques for the laser ablation of targeted cells in the early embryo of the brown alga Saccharina latissima.

Targeted Laser Ablation in the Embryo of Saccharina latissima

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