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Targeted Laser Ablation in the Embryo of Saccharina latissima
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发育生物学
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JoVE 杂志 发育生物学
Targeted Laser Ablation in the Embryo of Saccharina latissima

Targeted Laser Ablation in the Embryo of Saccharina latissima

DOI:
10.3791/63518-v

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06:30 min

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March 11, 2022

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, , ,
1UMR8227, CNRS / Sorbonne University, 2Univ Rennes, CNRS, Inserm, Biosit UAR 3480 US_S 018, MRic Core Facility

Chapters

  • 00:04Introduction
  • 00:47Image Acquisition for Selecting Embryos for Ablation and Monitoring Subsequent Growth
  • 01:31Laser Calibration
  • 02:48Laser Ablation
  • 04:21Monitoring the Growth of Irradiated Embryos
  • 04:49Results: Effect of Local Cellular Laser Ablation on the Saccharina latissima Embryo
  • 05:46Conclusion

Summary

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The destruction of specific cells in the embryo is a powerful tool for studying cellular interactions involved in cell fate. The present protocol describes techniques for the laser ablation of targeted cells in the early embryo of the brown alga Saccharina latissima.

Tags

Keywords: Laser AblationSaccharina LatissimaEmbryogenesisBrown AlgaeCell FateCell InteractionImagingTime-lapse MicroscopyProtocol

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