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Targeted Laser Ablation in the Embryo of Saccharina latissima
JoVE Revista
Biología del desarrollo
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JoVE Revista Biología del desarrollo
Targeted Laser Ablation in the Embryo of Saccharina latissima
DOI:

06:30 min

March 11, 2022

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Capítulos

  • 00:04Introduction
  • 00:47Image Acquisition for Selecting Embryos for Ablation and Monitoring Subsequent Growth
  • 01:31Laser Calibration
  • 02:48Laser Ablation
  • 04:21Monitoring the Growth of Irradiated Embryos
  • 04:49Results: Effect of Local Cellular Laser Ablation on the Saccharina latissima Embryo
  • 05:46Conclusion

Summary

Traducción Automática

The destruction of specific cells in the embryo is a powerful tool for studying cellular interactions involved in cell fate. The present protocol describes techniques for the laser ablation of targeted cells in the early embryo of the brown alga Saccharina latissima.

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