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Genetics

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JoVE Journal Genetics
Site-Directed φC31-medieret integration og kassetteudveksling i anopheles vektorer af malaria
 
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Site-Directed φC31-medieret integration og kassetteudveksling i anopheles vektorer af malaria

Article DOI: 10.3791/62146-v • 09:38 min • February 2nd, 2021
February 2nd, 2021 •
1,2, 1, 1, 2,3
1Vector Biology Department, Liverpool School of Tropical Medicine, 2Department of Microbiology & Molecular Genetics, University of California, 3Department of Molecular Biology & Biochemistry, University of California

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Protokollen beskriver, hvordan man opnår stedstyrede modifikationer i genomet af Anopheles malaria myg ved hjælp af φC31-systemet . De beskrevne modifikationer omfatter både integration og udveksling af transgene kassetter i genomet af attP-bærende dockinglinjer.

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Genetik udgave 168 Anopheles φC31 site-directed docking integration kassetteudveksling myg transgen
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