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1UCLA AIDS Institute,University of California at Los Angeles (UCLA), 2Department of Microbiology, Immunology, & Molecular Genetics,University of California at Los Angeles (UCLA), 3Departments of Biomathematics and Mathematics,University of California at Los Angeles (UCLA), 4Personalized Genomic Medicine Research Center, Division of Strategic Research Groups,Korea Research Institute of Bioscience and Biotechnology, 5Department of Medicine,University of California at Los Angeles (UCLA), 6Department of Veterinary Biosciences, College of Veterinary Medicine,The Ohio State University (OSU)
Chapitres
- 00:05Titre
- 01:11LTR-specific Biotin-primer Extension
- 02:53Rsal and CviQI Digestion and Blunt-ending
- 04:12Straptavidin Bead Binding
- 05:31Linker Ligation
- 06:39Pre-amplification of Both Left and Right Junction DNA
- 08:15Left- and Right-junction-specific Amplifications
- 10:23Results: The IS Bidirectional Assay Improves Detection Rates and Sequence Quantification Accuracy
- 12:09Conclusion
Dette manuskript beskriver den eksperimentelle procedure og softwareanalyse for et tovejs integrationssite-assay, der samtidigt kan analysere opstrøms og nedstrøms vektor-værtsforbindelses-DNA. Tovejsede PCR-produkter kan anvendes til enhver downstream-sekventeringsplatform. De resulterende data er nyttige til en høj-gennemløbs kvantitativ sammenligning af integrerede DNA-mål.
