Journal
/
Génétique
/
Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example
Journal JoVE
Génétique
This content is Free Access.
Journal JoVE Génétique
Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

DOI:
10.3791/60906-v

•

05:45 min

•

March 11, 2020

•

, , ,
1ErasmusMC, Department of Viroscience, WHO Collaborating Centre for Arbovirus and Viral Hemorrhagic Fever Reference and Research,Erasmus University Medical Center

Chapitres

  • 00:04Introduction
  • 00:59Nanopore Data Consensus Sequence Generation
  • 02:33Nanopore Sequencing Error Profile Compensation
  • 03:54Results: Representative Nanopore Sequencing Error Rate Overview
  • 04:58Conclusion

Summary

Traduction automatique

Traduction automatique

We previously validated a protocol for amplicon-based whole genome Usutu virus (USUV) sequencing on a nanopore sequencing platform. Here, we describe the methods used in more detail and determine the error rate of the nanopore R10 flow cell.

Tags

Artificial IntelligenceAI Writing AssistantsCopywritingContent GenerationText-based ContentContent CreationAI-assisted WritingWriter’s BlockContent EfficiencyReal-time SequencingVirus Genetic SequencingNanopore SequencingUsutu VirusError RateRead CoverageSequence DataConsensus SequenceReference-based AlignmentMinimap2SamtoolsKMA

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

Separating Bacteria by Capsule Amount Using a Discontinuous Density Gradient

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Read Article