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肿瘤微环境下免疫细胞种群的可视化、定量和映射
Journal JoVE
Immunologie et infection
This content is Free Access.
Journal JoVE Immunologie et infection
Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment
DOI:

11:00 min

March 25, 2020

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Chapitres

  • 00:05Introduction
  • 01:03Heat-Induced Antigen Retrieval
  • 01:46Nonspecific Binding Blocking
  • 02:28Immunofluorescence Labeling
  • 03:36Image Alignment
  • 04:36Tissue Detection
  • 05:35Tissue Segmentation
  • 06:28Cell of Interest (COI) Identification and Quantification
  • 07:15Tissue Heatmapping
  • 08:10Results: Representative Visualization, Quantification, and Mapping of Cell Populations of Interest in the Tumor Microenvironment
  • 10:02Conclusion

Summary

Traduction automatique

在这里,我们描述了一种简单易懂的策略,用于在正式固定石蜡嵌入肿瘤组织部分中可视化、量化和映射免疫细胞。该方法将现有的成像和数字分析技术与扩展成像检测的多路复用能力和多参数分析相结合。

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