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Direcionando drogas para macrófagos de zebrafish larval injetando liposós carregados de drogas
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Immunologie et infection
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Journal JoVE Immunologie et infection
Targeting Drugs to Larval Zebrafish Macrophages by Injecting Drug-Loaded Liposomes

Direcionando drogas para macrófagos de zebrafish larval injetando liposós carregados de drogas

7,456 Views

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11:31 min

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February 18, 2020

DOI:

10.3791/60198-v

DOI:
10.3791/60198-v

•

11:31 min
February 18, 2020

•

7 Views
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1Department of Molecular Medicine and Pathology, Faculty of Medical and Health Sciences,University of Auckland, 2School of Pharmacy, Faculty of Medical and Health Sciences,University of Auckland, 3School of Medicine, Faculty of Medical and Health Sciences,University of Auckland

Summary

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Aqui, descrevemos a síntese de liposós carregados de drogas e sua microinjeção em zebrafish larval com o propósito de direcionar o fornecimento de drogas para células de linhagem de macrófagos.

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LiposomesMacrophagesZebrafishDrug DeliveryMitochondrial Reactive Oxygen SpeciesPoloxamer 188ExtrusionUltracentrifugationLive Cell Imaging

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Cite this Article

Linnerz, T., Kanamala, M., Astin, J. W., Dalbeth, N., Wu, Z., Hall, C. J. Targeting Drugs to Larval Zebrafish Macrophages by Injecting Drug-Loaded Liposomes. J. Vis. Exp. (156), e60198, doi:10.3791/60198 (2020).

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