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Affinity Purification of Influenza Virus Ribonucleoprotein Complexes from the Chromatin of Infected Cells
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Affinity Purification of Influenza Virus Ribonucleoprotein Complexes from the Chromatin of Infected Cells
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11:20 min
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June 03, 2012
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Summary
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Influenza viruses replicate their RNA genome in association with host-cell chromatin. Here, we present a method to purify intact viral ribonucleoprotein complexes from the chromatin of infected cells. Purified viral complexes can be analyzed by both Western blot and primer extension of protein and RNA content, respectively.
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Chase, G. P., Schwemmle, M. Affinity Purification of Influenza Virus Ribonucleoprotein Complexes from the Chromatin of Infected Cells. J. Vis. Exp. (64), e4028, doi:10.3791/4028 (2012).
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