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An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets
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Immunologie et infection
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Journal JoVE Immunologie et infection
An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets

An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets

14,852 Views

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09:09 min

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April 18, 2016

DOI:

10.3791/53824-v

DOI:
10.3791/53824-v

•

09:09 min
April 18, 2016

•

1 Views
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1Department of Microbiology and Immunology,Albert Einstein College of Medicine, 2Department of Medicine (Rheumatology),Albert Einstein College of Medicine

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Mouse Dendritic CellsDC SubsetsFlt-3 LigandB16 MelanomaC57 Black 6 MiceSpleenCollagenaseDNaseCell StrainerRed Blood Cell LysisFC BlockPlasma Cytoid DCsBiotin-labeled Antibody CocktailCell Sorting

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Arora, P., Porcelli, S. A. An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets. J. Vis. Exp. (110), e53824, doi:10.3791/53824 (2016).

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