/
/
Formation of Human Periodontal Ligament Cell Spheroids on Chitosan Films
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Formation of Human Periodontal Ligament Cell Spheroids on Chitosan Films
5,221 Views
•
04:14 min
•
June 19, 2019
DOI:
1Department of Periodontology, School & Hospital of Stomatology,Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, 2Department of Periodontology,Dental Diseases Prevention & Treatment Center of Jiading District, 3College of Materials Science and Engineering,Tongji University
Summary
Automatically generated
Here, we present protocols of culturing human periodontal ligament (PDL) cell spheroids by chitosan films. The culture of three-dimensional (3D) cellular spheroids provides an alternative to conventional tissue culture polystyrene (TCPS) culture system.
Read Article
Cite this Article
Yan, X., Ran, X., Xia, S., Yang, Y., Zhou, M., Yuan, C., Luo, L. Formation of Human Periodontal Ligament Cell Spheroids on Chitosan Films. J. Vis. Exp. (148), e59855, doi:10.3791/59855 (2019).
Copy