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Formation of Human Periodontal Ligament Cell Spheroids on Chitosan Films
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Formation of Human Periodontal Ligament Cell Spheroids on Chitosan Films
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04:14 min
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June 19, 2019
DOI:
1Department of Periodontology, School & Hospital of Stomatology,Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, 2Department of Periodontology,Dental Diseases Prevention & Treatment Center of Jiading District, 3College of Materials Science and Engineering,Tongji University
Summary
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Here, we present protocols of culturing human periodontal ligament (PDL) cell spheroids by chitosan films. The culture of three-dimensional (3D) cellular spheroids provides an alternative to conventional tissue culture polystyrene (TCPS) culture system.
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Yan, X., Ran, X., Xia, S., Yang, Y., Zhou, M., Yuan, C., Luo, L. Formation of Human Periodontal Ligament Cell Spheroids on Chitosan Films. J. Vis. Exp. (148), e59855, doi:10.3791/59855 (2019).
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