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Formation of Human Periodontal Ligament Cell Spheroids on Chitosan Films
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发育生物学
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JoVE 杂志 发育生物学
Formation of Human Periodontal Ligament Cell Spheroids on Chitosan Films

Formation of Human Periodontal Ligament Cell Spheroids on Chitosan Films

DOI:
10.3791/59855-v

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04:14 min

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June 19, 2019

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, , , , , ,
1Department of Periodontology, School & Hospital of Stomatology,Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, 2Department of Periodontology,Dental Diseases Prevention & Treatment Center of Jiading District, 3College of Materials Science and Engineering,Tongji University

Chapters

  • 00:04Title
  • 00:33Preparation of Chitosan Films
  • 01:33Cell Seeding
  • 03:00Results: The Morphology and Viability of PDL Cellular Spheroids on Chitosan Films
  • 03:47Conclusion

Summary

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Here, we present protocols of culturing human periodontal ligament (PDL) cell spheroids by chitosan films. The culture of three-dimensional (3D) cellular spheroids provides an alternative to conventional tissue culture polystyrene (TCPS) culture system.

Tags

Periodontal Ligament CellsCell SpheroidsChitosan FilmsCell CultureOsteogenic DifferentiationCell Seeding DensityCell Morphology

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