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Isolation and Quantitative Evaluation of Brush Cells from Mouse Tracheas
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Chapitres
- 00:04Titre
- 01:24Preparation of Reagents
- 02:34Dissection of Mouse Trachea
- 04:47Tracheal Epithelial Digestion
- 08:31Results: Analysis of the Isolated Brush Cells from Mouse Tracheas
- 09:57Conclusion
Brush cells are rare cholinergic chemosensory epithelial cells found in the naïve mouse trachea. Due to their limited numbers, ex vivo evaluation of their functional role in airway immunity and remodeling is challenging. We describe a method for isolation of tracheal brush cells by flow cytometry.
