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Immunologie et infection
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小鼠特拉切斯刷细胞的分离与定量评价
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Immunologie et infection
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Journal JoVE Immunologie et infection
Isolation and Quantitative Evaluation of Brush Cells from Mouse Tracheas
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小鼠特拉切斯刷细胞的分离与定量评价

7,007 Views

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10:25 min

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June 12, 2019

DOI:

10.3791/59496-v

DOI:
10.3791/59496-v

•

10:25 min
June 12, 2019

•

2 Views
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1Division of Rheumatology, Allergy and Immunology, Brigham and Women’s Hospital,Harvard Medical School

Summary

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刷细胞是罕见的胆碱能化学感觉上皮细胞发现在天真的小鼠气管。由于其数量有限,其功能在气道免疫和改造中的作用的体外评估具有挑战性。我们描述了一种通过流式细胞测定分离气管刷细胞的方法。

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Cite this Article

Ualiyeva, S., Yoshimoto, E., Barrett, N. A., Bankova, L. G. Isolation and Quantitative Evaluation of Brush Cells from Mouse Tracheas. J. Vis. Exp. (148), e59496, doi:10.3791/59496 (2019).

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