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Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells
Journal JoVE
Génétique
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Journal JoVE Génétique
Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells

Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells

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11:16 min

February 15, 2019

DOI:

11:16 min
February 15, 2019

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Summary

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The goal of this protocol is to isolate nonhuman primate CD34+ cells from primed bone marrow, to gene-modify these cells with lentiviral vectors, and to prepare a product for infusion into the autologous host. The total protocol length is approximately 48 h.

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