Journal
/
Genética
/
Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells
JoVE Revista
Genética
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Genética
Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells

Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells

7,442 Views

•

11:16 min

•

February 15, 2019

DOI:

10.3791/58933-v

DOI:
10.3791/58933-v

•

11:16 min
February 15, 2019

•

5 Views
, , , , , , ,
1Stem Cell and Gene Therapy Program,Fred Hutchinson Cancer Research Center, 2Department of Medicine,University of Washington, 3Department of Pathology,University of Washington

Summary

Automatically generated

The goal of this protocol is to isolate nonhuman primate CD34+ cells from primed bone marrow, to gene-modify these cells with lentiviral vectors, and to prepare a product for infusion into the autologous host. The total protocol length is approximately 48 h.

Explore More Videos

Nonhuman PrimateHematopoietic Stem CellsProgenitor CellsGene TherapyBone MarrowCell IsolationCD34Magnetic Cell SortingFlow CytometryCell Culture

Read Article

Cite this Article

Radtke, S., Perez, A. M., Venkataraman, R., Reddy, S., Haworth, K. G., Humbert, O., Kiem, H., Peterson, C. W. Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells. J. Vis. Exp. (144), e58933, doi:10.3791/58933 (2019).

Download .ris file

Copy

Share Video

.

Copy