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Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells
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JoVE Journal Genetik
Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells

Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells

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11:16 min

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February 15, 2019

DOI:

10.3791/58933-v

DOI:
10.3791/58933-v

•

11:16 min
February 15, 2019

•

5 Views
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1Stem Cell and Gene Therapy Program,Fred Hutchinson Cancer Research Center, 2Department of Medicine,University of Washington, 3Department of Pathology,University of Washington

Summary

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The goal of this protocol is to isolate nonhuman primate CD34+ cells from primed bone marrow, to gene-modify these cells with lentiviral vectors, and to prepare a product for infusion into the autologous host. The total protocol length is approximately 48 h.

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Nonhuman PrimateHematopoietic Stem CellsProgenitor CellsGene TherapyBone MarrowCell IsolationCD34Magnetic Cell SortingFlow CytometryCell Culture

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Cite this Article

Radtke, S., Perez, A. M., Venkataraman, R., Reddy, S., Haworth, K. G., Humbert, O., Kiem, H., Peterson, C. W. Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells. J. Vis. Exp. (144), e58933, doi:10.3791/58933 (2019).

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