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Isolation of Primary Murine Skeletal Muscle Microvascular Endothelial Cells
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Neurosciences
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Journal JoVE Neurosciences
Isolation of Primary Murine Skeletal Muscle Microvascular Endothelial Cells

Isolation of Primary Murine Skeletal Muscle Microvascular Endothelial Cells

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11:57 min

March 06, 2019

DOI:

11:57 min
March 06, 2019

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Summary

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Microvascular endothelial cells of skeletal muscles (MMEC) shape the inner wall of muscle capillaries and regulate both, exchange of fluids/molecules and migration of (immune) cells between muscle tissue and blood. Isolation of primary murine MMEC, as described here, enables comprehensive in vitro investigations of the "myovascular unit".

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