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Isolation of Primary Murine Skeletal Muscle Microvascular Endothelial Cells
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神经科学
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JoVE 杂志 神经科学
Isolation of Primary Murine Skeletal Muscle Microvascular Endothelial Cells

Isolation of Primary Murine Skeletal Muscle Microvascular Endothelial Cells

DOI:
10.3791/58901-v

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11:57 min

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March 06, 2019

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, , , ,
1Institute for Translational Neurology and Neurology Clinic,University of Muenster

Chapters

  • 00:04Title
  • 00:47Isolation of Primary Murine MMEC
  • 08:03Primary Murine MMEC Purification
  • 09:04Results: Analysis of the Isolated Primary Murine Skeletal Muscle Microvascular Endothelial Cells
  • 11:20Conclusion

Summary

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Microvascular endothelial cells of skeletal muscles (MMEC) shape the inner wall of muscle capillaries and regulate both, exchange of fluids/molecules and migration of (immune) cells between muscle tissue and blood. Isolation of primary murine MMEC, as described here, enables comprehensive in vitro investigations of the "myovascular unit".

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Primary MurineSkeletal MuscleMicrovascular Endothelial CellsIsolationCell CultureDigestion

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