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Isolation of Primary Murine Skeletal Muscle Microvascular Endothelial Cells
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Isolation of Primary Murine Skeletal Muscle Microvascular Endothelial Cells
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March 06, 2019
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Summary
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Microvascular endothelial cells of skeletal muscles (MMEC) shape the inner wall of muscle capillaries and regulate both, exchange of fluids/molecules and migration of (immune) cells between muscle tissue and blood. Isolation of primary murine MMEC, as described here, enables comprehensive in vitro investigations of the "myovascular unit".
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Müntefering, T., Michels, A. P., Pfeuffer, S., Meuth, S. G., Ruck, T. Isolation of Primary Murine Skeletal Muscle Microvascular Endothelial Cells. J. Vis. Exp. (145), e58901, doi:10.3791/58901 (2019).
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