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Isolation of Primary Murine Skeletal Muscle Microvascular Endothelial Cells
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JoVE Journal Neurowissenschaften
Isolation of Primary Murine Skeletal Muscle Microvascular Endothelial Cells

Isolation of Primary Murine Skeletal Muscle Microvascular Endothelial Cells

DOI:
10.3791/58901-v

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11:57 min

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March 06, 2019

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, , , ,
1Institute for Translational Neurology and Neurology Clinic,University of Muenster

Kapitel

  • 00:04Titel
  • 00:47Isolation of Primary Murine MMEC
  • 08:03Primary Murine MMEC Purification
  • 09:04Results: Analysis of the Isolated Primary Murine Skeletal Muscle Microvascular Endothelial Cells
  • 11:20Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Microvascular endothelial cells of skeletal muscles (MMEC) shape the inner wall of muscle capillaries and regulate both, exchange of fluids/molecules and migration of (immune) cells between muscle tissue and blood. Isolation of primary murine MMEC, as described here, enables comprehensive in vitro investigations of the "myovascular unit".

Tags

Primary MurineSkeletal MuscleMicrovascular Endothelial CellsIsolationCell CultureDigestion

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