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小鼠边缘区 b 细胞剪切流诱导迁移的体外分析
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Immunologie et infection
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Journal JoVE Immunologie et infection
Analysis of Shear Flow-induced Migration of Murine Marginal Zone B Cells In Vitro
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小鼠边缘区 b 细胞剪切流诱导迁移的体外分析

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08:31 min

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November 26, 2018

DOI:

10.3791/58759-v

DOI:
10.3791/58759-v

•

08:31 min
November 26, 2018

•

1 Views
, , , ,
1Institute of Biochemistry and Cell Biology,OVGU University of Magdeburg

Summary

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边缘区 b 细胞 (mzb) 通过调整其迁移路径的方向来响应剪切流的力。该协议演示了如何使用流体单元、泵、显微镜成像系统和自由软件记录和分析迁移。

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Cite this Article

Tedford, K., Tech, L., Steiner, M., Korthals, M., Fischer, K. Analysis of Shear Flow-induced Migration of Murine Marginal Zone B Cells In Vitro. J. Vis. Exp. (141), e58759, doi:10.3791/58759 (2018).

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