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Measuring Liver Mitochondrial Oxygen Consumption and Proton Leak Kinetics to Estimate Mitochondrial Respiration in Holstein Dairy Cattle
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Measuring Liver Mitochondrial Oxygen Consumption and Proton Leak Kinetics to Estimate Mitochondrial Respiration in Holstein Dairy Cattle

Measuring Liver Mitochondrial Oxygen Consumption and Proton Leak Kinetics to Estimate Mitochondrial Respiration in Holstein Dairy Cattle

10,895 Views

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08:29 min

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November 30, 2018

DOI:

10.3791/58387-v

DOI:
10.3791/58387-v

•

08:29 min
November 30, 2018

•

6 Views
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1Veterinary Medicine Teaching and Research Center,University of California Davis, 2Archer Daniels Midland, 3Department of Molecular Biosciences,University of California Davis

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Mitochondrial Oxygen ConsumptionProton Leak KineticsMitochondrial RespirationDairy CattleEnergeticsMitochondrial IsolationLiver SampleHomogenizationCentrifugationProtein ConcentrationOxygen Consumption AssayRespiration ChamberOxygraph System

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Rossow, H. A., Acetoze, G., Champagne, J., Ramsey, J. J. Measuring Liver Mitochondrial Oxygen Consumption and Proton Leak Kinetics to Estimate Mitochondrial Respiration in Holstein Dairy Cattle. J. Vis. Exp. (141), e58387, doi:10.3791/58387 (2018).

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