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乙型肝炎体外感染形成的低拷贝数综合病毒 dna 的检测
Journal JoVE
Immunologie et infection
This content is Free Access.
Journal JoVE Immunologie et infection
Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection
DOI:

11:14 min

November 07, 2018

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Chapitres

  • 00:04Titre
  • 00:52Cell Infection and DNA Extraction
  • 02:42Inversion of DNA
  • 05:55Nested PCR
  • 08:30PCR Product Isolation and Gel Extraction
  • 09:37Results: Agarose Gel Electrophoresis and BLAST Analysis of InvPCR Product Sequences
  • 10:24Conclusion

Summary

Traduction automatique

我们这里描述了 hbv dna 的体外生成通过乙型肝炎病毒感染系统和高度敏感的检测其 (1-2 拷贝) 整合使用反向嵌套 pcr。

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