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Immunology and Infection

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JoVE Journal Immunology and Infection
Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection
 

Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection

Article DOI: 10.3791/58202-v • 11:14 min • November 7th, 2018
November 7th, 2018 •
1,2, 1,3
1Department of Infectious Diseases, Molecular Virology, Heidelberg University Hospital, 2Gastroenterology and Liver Research Laboratory, Ingham Institute, 3German Center for Infection Research (DZIF)

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We describe here the in vitro generation of HBV DNA via a Hepatitis B virus infection system and the highly sensitive detection of its (1–2 copies) integration using inverse nested PCR.

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Low Copy Number Integrated Viral DNA Hepatitis B In Vitro Infection HPV DNA Integration Virological Factors Cheap Easy Sensitive HBV Integration HIV HTLV-1 HPV Cell Infection Culture Medium Tenofovir Disoproxil Lamivudine Inverse PCR
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