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Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection

Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection

17,976 Views

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11:14 min

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November 07, 2018

DOI:

10.3791/58202-v

DOI:
10.3791/58202-v

•

11:14 min
November 07, 2018

•

3 Views
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1Department of Infectious Diseases, Molecular Virology,Heidelberg University Hospital, 2Gastroenterology and Liver Research Laboratory,Ingham Institute, 3German Center for Infection Research (DZIF)

Summary

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We describe here the in vitro generation of HBV DNA via a Hepatitis B virus infection system and the highly sensitive detection of its (1–2 copies) integration using inverse nested PCR.

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HBV DNA IntegrationHBV VirologyLow Copy NumberIn Vitro HBV InfectionInverse PCRCell CultureVirus IntegrationDNA ExtractionDNA DigestionQuantitative Analysis

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Cite this Article

Tu, T., Urban, S. Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection. J. Vis. Exp. (141), e58202, doi:10.3791/58202 (2018).

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