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高通量的高效液氧活化病毒膜融合检测 HIV-1 病毒膜融合抑制剂的研究
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Immunologie et infection
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Journal JoVE Immunologie et infection
A High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion
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高通量的高效液氧活化病毒膜融合检测 HIV-1 病毒膜融合抑制剂的研究

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07:22 min

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August 14, 2018

DOI:

10.3791/58074-v

DOI:
10.3791/58074-v

•

07:22 min
August 14, 2018

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7 Views
, , , , ,
1Division of Infectious Diseases, Department of Medicine,Icahn School of Medicine at Mount Sinai, Immunology Institute, 2Department of Biology,New Jersey City University

Tags

Cre-LoxViral Membrane FusionHIV-1Drug DiscoveryHigh-throughput ScreeningHIV Fusion InhibitorsBiosafetyJurkat RG Reporter CellsPuromycinCell-to-cell Virus TransmissionGag-iCre DNAElectroporationFicoll

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Esposito, A. M., Soare, A. Y., Patel, F., Satija, N., Chen, B. K., Swartz, T. H. A High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion. J. Vis. Exp. (138), e58074, doi:10.3791/58074 (2018).

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