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In Vivo Microdialysis Method to Collect Large Extracellular Proteins from Brain Interstitial Fluid with High-molecular Weight Cut-off Probes
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Journal JoVE Neurosciences
In Vivo Microdialysis Method to Collect Large Extracellular Proteins from Brain Interstitial Fluid with High-molecular Weight Cut-off Probes

In Vivo Microdialysis Method to Collect Large Extracellular Proteins from Brain Interstitial Fluid with High-molecular Weight Cut-off Probes

DOI:
10.3791/57869-v

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08:17 min

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September 26, 2018

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1Department of Neuropathology, Graduate School of Medicine,University of Tokyo

Chapitres

  • 00:04Titre
  • 00:42Guide Cannula Implantation
  • 04:17Microdialysis Setup
  • 07:24Results: Representative Interstitial Fluid (ISF) Tau Changes Upon Picrotoxin Reverse Microdialysis
  • 07:52Conclusion

Summary

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In vivo microdialysis has enabled collection of molecules present in brain interstitial fluid (ISF) from awake, freely-behaving animals. In order to analyze relatively large molecules in ISF, the current article specifically focuses on the microdialysis protocol using probes with high molecular weight cut off membranes.

Tags

In Vivo MicrodialysisBrain Interstitial FluidHigh-molecular Weight Cut-off ProbesNeuroscience ResearchSignal TransductionSubstrate TransportWaste ClearanceExtracellular MoleculesAwake Freely-moving AnimalsStereotaxic ApparatusGuide CannulaDental CementMicrodialysis Probe

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