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Neuroscience

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JoVE Journal Neuroscience
In Vivo Microdialysis Method to Collect Large Extracellular Proteins from Brain Interstitial Fluid with High-molecular Weight Cut-off Probes
 

In Vivo Microdialysis Method to Collect Large Extracellular Proteins from Brain Interstitial Fluid with High-molecular Weight Cut-off Probes

Article DOI: 10.3791/57869-v • 08:17 min • September 26th, 2018
September 26th, 2018 •
1
1Department of Neuropathology, Graduate School of Medicine, University of Tokyo

Kapitel

Summary
Automatic Translation

In vivo microdialysis has enabled collection of molecules present in brain interstitial fluid (ISF) from awake, freely-behaving animals. In order to analyze relatively large molecules in ISF, the current article specifically focuses on the microdialysis protocol using probes with high molecular weight cut off membranes.

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Keywords: In Vivo Microdialysis Brain Interstitial Fluid High-molecular Weight Cut-off Probes Neuroscience Research Signal Transduction Substrate Transport Waste Clearance Extracellular Molecules Awake Freely-moving Animals Stereotaxic Apparatus Guide Cannula Dental Cement Microdialysis Probe
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