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基于纠错 DNA 和 RNA 测序的稀有事件检测方法

Journal JoVE
Génétique

This content is Free Access.

Journal JoVE Génétique

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

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基于纠错 DNA 和 RNA 测序的稀有事件检测方法

11,576 Views

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10:36 min

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August 03, 2018

DOI:

10.3791/57509-v

DOI:

10.3791/57509-v

•

10:36 min

August 03, 2018

•

4 Views

Wing H. Wong*^1,2, R. Spencer Tong*^1,2, Andrew L. Young^1,2, Todd E. Druley^1,2

¹Department of Pediatrics, Division of Hematology and Oncology,Washington University School of Medicine, ²Center for Genome Sciences and Systems Biology,Washington University School of Medicine

Summary

下一代测序 (0.5–2.0%) 是基因组特征的有力工具, 受平台高错误率的限制。我们描述了我们的错误纠正排序方法, 使我们能够避免的误差率和检测突变的变异等位基因分数罕见的0.0001。

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