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Using Mouse Oocytes to Assess Human Gene Function During Meiosis I
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Journal JoVE Génétique
Using Mouse Oocytes to Assess Human Gene Function During Meiosis I

Using Mouse Oocytes to Assess Human Gene Function During Meiosis I

DOI:
10.3791/57442-v

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11:13 min

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April 10, 2018

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, , ,
1IVI-RMA New Jersey, 2Jefferson College of Biomedical Sciences,Thomas Jefferson University, 3Department of Genetics,Rutgers, The State University of New Jersey

Chapitres

  • 00:04Titre
  • 01:00Molecular Cloning
  • 02:23Kinetochore-microtubule Attachment Assay
  • 04:57Chromosome Alignment Challenge Assay
  • 08:52Results: Assessment of Human Gene Function During Meiosis in Mouse Oocytes
  • 10:12Conclusion

Summary

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As the genetic variants associated with human disease begin to become uncovered, it is becoming increasingly important to develop systems with which to rapidly evaluate the biological significance of those identified variants. This protocol describes methods for evaluating human gene function during female meiosis I using mouse oocytes.

Tags

Mouse OocytesHuman Gene FunctionMeiosis IMaternal AneuploidyIn Vitro FertilizationExome SequencingCloningMutagenesisIn Vitro TranscriptionMicroinjectionMilrinoneMetaphase I

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