Journal
/
Neurosciences
/
Isolation of Cerebral Capillaries from Fresh Human Brain Tissue
Journal JoVE
Neurosciences
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Neurosciences
Isolation of Cerebral Capillaries from Fresh Human Brain Tissue

Isolation of Cerebral Capillaries from Fresh Human Brain Tissue

12,332 Views

•

06:35 min

•

September 12, 2018

DOI:

10.3791/57346-v

DOI:
10.3791/57346-v

•

06:35 min
September 12, 2018

•

3 Views
, , , , , , , ,
1Sanders-Brown Center on Aging, Department of Pharmacology and Nutritional Sciences,University of Kentucky, 2Department of Pharmaceutical Sciences, College of Pharmacy,University of Kentucky, 3Department of Neuroscience,University of Kentucky

Summary

Automatically generated

Isolated brain capillaries from human brain tissue can be used as a preclinical model to study barrier function under physiological and pathophysiological conditions. Here, we present an optimized protocol to isolate brain capillaries from fresh human brain tissue.

Explore More Videos

Cerebral CapillariesBlood-brain BarrierIsolationHomogenizationFicoll Density GradientFiltrationCentrifugation

Read Article

Cite this Article

Hartz, A. M., Schulz, J. A., Sokola, B. S., Edelmann, S. E., Shen, A. N., Rempe, R. G., Zhong, Y., Seblani, N. E., Bauer, B. Isolation of Cerebral Capillaries from Fresh Human Brain Tissue. J. Vis. Exp. (139), e57346, doi:10.3791/57346 (2018).

Download .ris file

Copy

Share Video

.

Copy