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Isolation of Cerebral Capillaries from Fresh Human Brain Tissue
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JoVE Journal Neurowissenschaften
Isolation of Cerebral Capillaries from Fresh Human Brain Tissue

Isolation of Cerebral Capillaries from Fresh Human Brain Tissue

12,332 Views

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06:35 min

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September 12, 2018

DOI:

10.3791/57346-v

DOI:
10.3791/57346-v

•

06:35 min
September 12, 2018

•

3 Views
, , , , , , , ,
1Sanders-Brown Center on Aging, Department of Pharmacology and Nutritional Sciences,University of Kentucky, 2Department of Pharmaceutical Sciences, College of Pharmacy,University of Kentucky, 3Department of Neuroscience,University of Kentucky

Summary

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Isolated brain capillaries from human brain tissue can be used as a preclinical model to study barrier function under physiological and pathophysiological conditions. Here, we present an optimized protocol to isolate brain capillaries from fresh human brain tissue.

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Hartz, A. M., Schulz, J. A., Sokola, B. S., Edelmann, S. E., Shen, A. N., Rempe, R. G., Zhong, Y., Seblani, N. E., Bauer, B. Isolation of Cerebral Capillaries from Fresh Human Brain Tissue. J. Vis. Exp. (139), e57346, doi:10.3791/57346 (2018).

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