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Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector
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March 28, 2018
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Using a pBIBAC-GW binary vector makes generating transgenic plants with intact single-copy insertions, an easy process. Here, a series of protocols is presented that guide the reader through the process of generating transgenic Arabidopsis plants, and testing the plants for intactness and copy number of the inserts.
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Tark-Dame, M., Weber, B., de Sain, M., Anggoro, D. T., Bader, R., Walmsley, A., Oka, R., Stam, M. Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector. J. Vis. Exp. (133), e57295, doi:10.3791/57295 (2018).
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