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Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector
JoVE 杂志
遗传学
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JoVE 杂志 遗传学
Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

12,163 Views

12:08 min

March 28, 2018

DOI:

12:08 min
March 28, 2018

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Summary

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Using a pBIBAC-GW binary vector makes generating transgenic plants with intact single-copy insertions, an easy process. Here, a series of protocols is presented that guide the reader through the process of generating transgenic Arabidopsis plants, and testing the plants for intactness and copy number of the inserts.

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