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Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector
Kapitel
- 00:04Titel
- 01:00Inserting Sequences of Interest into BIBAC-GW Binary Vector
- 02:37Arabidopsis Transformation
- 05:37Characterizing Transgenics for the Number and Integrity of T-DNA Integrations
- 08:19Results: Analysis of the Number of T-DNA Insertions in Plants Transformed with BIBAC-Bar-GW Carrying a Reporter Sequence
- 10:01Conclusion
Using a pBIBAC-GW binary vector makes generating transgenic plants with intact single-copy insertions, an easy process. Here, a series of protocols is presented that guide the reader through the process of generating transgenic Arabidopsis plants, and testing the plants for intactness and copy number of the inserts.
