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小鼠肝灌注肝细胞和正弦内皮细胞的纯化

Journal JoVE
Biochimie

This content is Free Access.

Journal JoVE Biochimie

Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion

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小鼠肝灌注肝细胞和正弦内皮细胞的纯化

DOI:

10.3791/56993-v

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08:54 min

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February 12, 2018

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Fatima Cabral, Colton M. Miller, Katrina M. Kudrna, Blake E. Hass, Jocelyn G. Daubendiek, Brianna M. Kellar, Edward N. Harris

¹Department of Biochemistry,University of Nebraska

Chapitres

00:04Titre
00:45Catheter Placement
02:46Liver Perfusion and Hepatocyte Purification
04:39Sinusoidal Endothelial Cell (SEC) Purification
07:18Results: Representative SEC Purification Analyses
08:24Conclusion

Summary

Traduction automatique

该协议的目的是获得高生存能力和高产量的肝细胞和正弦内皮细胞。这是通过灌注肝与 IV 型胶原酶溶液通过门静脉, 其次是差异离心获得肝细胞和正弦内皮细胞。

Tags

Mouse Liver Perfusion Hepatocyte Purification Sinusoidal Endothelial Cell Purification Liver Cell Populations Portal Vein Catheterization Liver Digestion Liver Cell Viability Hepatocyte Harvesting Buffer Solutions

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