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Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion

Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion

48,641 Views

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08:54 min

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February 12, 2018

DOI:

10.3791/56993-v

DOI:
10.3791/56993-v

•

08:54 min
February 12, 2018

•

33 Views
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1Department of Biochemistry,University of Nebraska

Tags

Mouse Liver PerfusionHepatocyte PurificationSinusoidal Endothelial Cell PurificationLiver Cell PopulationsPortal Vein CatheterizationLiver DigestionLiver Cell ViabilityHepatocyte HarvestingBuffer Solutions

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Cabral, F., Miller, C. M., Kudrna, K. M., Hass, B. E., Daubendiek, J. G., Kellar, B. M., Harris, E. N. Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion. J. Vis. Exp. (132), e56993, doi:10.3791/56993 (2018).

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