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Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion
章
- 00:04標題
- 00:45Catheter Placement
- 02:46Liver Perfusion and Hepatocyte Purification
- 04:39Sinusoidal Endothelial Cell (SEC) Purification
- 07:18Results: Representative SEC Purification Analyses
- 08:24Conclusion
The goal of this protocol is to obtain high viability and high yield of hepatocytes and sinusoidal endothelial cells from liver. This is accomplished by perfusing the liver with a type IV collagenase solution via the portal vein, followed by differential centrifugation to obtain hepatocytes and sinusoidal endothelial cells.
