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Journal / Genetics / Immunostaining for DNA Modifications:…
JoVE Journal
Genetics

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JoVE Journal Genetics
Immunostaining for DNA Modifications
 

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Article DOI: 10.3791/56318-v • 09:42 min • September 7th, 2017
September 7th, 2017 •
*1, *1, 1, 1, 1, 2, 2, 1, 3, 1
1Division of Cancer and Stem Cells, School of Medicine, Centre for Biomolecular Sciences, University of Nottingham, 2School of Life Sciences Imaging (SLIM), School of Life Sciences, University of Nottingham, 3Children's Brain Tumour Research Centre, School of Medicine, QMC, University of Nottingham
* These authors contributed equally

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résumé
Automatic Translation

Newly discovered oxidized forms of 5-methylcytosine (oxi-mCs), 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) may represent distinct DNA modifications with unique functional roles. Here a semi-quantitative workflow for visualization of oxi-mCs' spatial distribution, signal intensity profiling and colocalization is described.

Tags

Immunostaining DNA Modifications Computational Analysis Confocal Images Spatial Distribution Signal Intensity Epigenetics Nuclear Localisation Model Systems Functional Roles Biological Systems Epitopes Immunohistochemistry LSM Formatted Files Confocal Microscopy Image Intensity Profiles Laser Power Gain Consistency Graphical Controls Rectangle Tool Region Of Interest
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