Journal
/
Génétique
/
Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
Journal JoVE
Génétique
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Génétique
Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

8,016 Views

•

09:04 min

•

September 25, 2019

DOI:

10.3791/60085-v

DOI:
10.3791/60085-v

•

09:04 min
September 25, 2019

•

3 Views
, , ,
1Center for Cellular and Molecular Therapeutics,The Children’s Hospital of Philadelphia, 2Department of Pathology and Laboratory Medicine,The Children’s Hospital of Philadelphia, 3Department of Pathology and Laboratory Medicine,University of Pennsylvania

Summary

Automatically generated

This protocol provides a method to facilitate the generation of defined heterozygous or homozygous nucleotide changes using CRISPR-CAS9 in human pluripotent stem cells.

Explore More Videos

CRISPR-Cas9Human Pluripotent Stem CellsIsogenic LinesHeterozygous MutationsGene FunctionDisease ModelingColony PickingFluorescence-activated Cell SortingMEFsROCK Inhibitor

Read Article

Cite this Article

Cardenas-Diaz, F. L., Maguire, J. A., Gadue, P., French, D. L. Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells. J. Vis. Exp. (151), e60085, doi:10.3791/60085 (2019).

Download .ris file

Copy

Share Video

.

Copy