Journal
/
Biologie du développement
/
Subcutaneous Neurotrophin 4 Infusion Using Osmotic Pumps or Direct Muscular Injection Enhances Aging Rat Laryngeal Muscles
Journal JoVE
Biologie du développement
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie du développement
Subcutaneous Neurotrophin 4 Infusion Using Osmotic Pumps or Direct Muscular Injection Enhances Aging Rat Laryngeal Muscles

Subcutaneous Neurotrophin 4 Infusion Using Osmotic Pumps or Direct Muscular Injection Enhances Aging Rat Laryngeal Muscles

DOI:
10.3791/55837-v

•

05:50 min

•

June 13, 2017

•

, , ,
1Department of Rehabilitation Sciences,University of Kentucky, 2Department of Physiology,University of Kentucky

Chapitres

  • 00:05Titre
  • 01:10Osmotic Pump Implantation
  • 02:49Direct Neurotrophin 4 (NTF4) Injection and Visualization
  • 04:42Results: Representative Effects of NTF4 Administration on Rat Laryngeal Muscle Fiber Size, Innervation, and Neuromuscular Junction Formation
  • 05:09Conclusion

Summary

Traduction automatique

Traduction automatique

Here, we present a protocol to describe the use of neurotrophin 4 (NTF4) systemically and directly to remodel rat aging laryngeal muscles.

Tags

SubcutaneousNeurotrophin 4Osmotic PumpsMuscular InjectionAgingLaryngeal MusclesVocal FunctionMuscle RemodelingMuscle AtrophyOtolaryngologySurgical ProcedureAseptic TechniqueAnesthesiaRat Model

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Isolation and Characterization of Satellite Cells from Rat Head Branchiomeric Muscles

Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids

Preparation of Rat Oligodendrocyte Progenitor Cultures and Quantification of Oligodendrogenesis Using Dual-infrared Fluorescence Scanning

Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues

Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning

Quantifying Branching Density in Rat Mammary Gland Whole-mounts Using the Sholl Analysis Method

Induction and Validation of Cellular Senescence in Primary Human Cells

Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence

Adult Zebrafish Injury Models to Study the Effects of Prednisolone in Regenerating Bone Tissue

Direct Lineage Reprogramming of Adult Mouse Fibroblast to Erythroid Progenitors

Read Article